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KMID : 0613820070170111482
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Journal of Life Science
2007 Volume.17 No. 11 p.1482 ~ p.1487
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Genetic Diversity and Phylogenetic Relationship of Genus Phyllostachys by ISSR Markers
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Lee Song-Jin
Huh Man-Kyu
Huh Hong-Wook
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Abstract
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Four taxa of the genus Phyllostachys were analysed with PCR-based molecular inter-simple sequence repeat (ISSR) determine markers to determine their phylogenetic relationships. Many species of this genus are regarded as economical and ecologically important in the world. With the nine primers screened, ISSR assay generated a total of 64 reproducible bands. Analysis of ISSR from individual plants of genus Phyllostachys resulted in 43 polymorphic bands with 70.49%. When species were grouped by four taxa, within group diversity was 0.092 (Hs), while among group diversity was 0.499 (Gst) on a per locus basis. The estimated gene flow (Nm) between the pairs of species was 0.559. Hence, we can expect weak or low gene flow among species. Total mean genetic diversity (HT) for four taxa was 0.291. The ISSR data allowed us to resolve well-supported clades in the genus Phyllostachys. The four taxa of the genus Phyllostachys analyzed were distinctly related to a monophyletic.
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KEYWORD
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Genus Phyllostachys, ISSR, phylogenetic relationships
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